Review



ppcrscript  (Agilent technologies)


Bioz Verified Symbol Agilent technologies is a verified supplier
Bioz Manufacturer Symbol Agilent technologies manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Agilent technologies ppcrscript
    Ppcrscript, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/pm24841577-73-18-19
    Average 90 stars, based on 1 article reviews
    ppcrscript - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: The enhanced probasin promoter, ARR 2 PB (courtesy of Dr. Robert Matusik) [ 34 , 35 ], was cloned into the MCS of the pBSIISK subcloning vector (Stratagene, La Jolla, CA) to construct pBSIISK-ARR 2 PB. .. Mouse KC, from the Kozak secretion signal through the penultimate amino acid, was PCR amplified from the IMAGE consortium clone {"type":"entrez-nucleotide","attrs":{"text":"BC037997","term_id":"23958859","term_text":"BC037997"}} BC037997 (Open BioSystems, Huntsville, AL), gel-purified (GeneClean kit, Qbiogene, Irvine, CA), shuttled through pPCRScript according to the manufacturer’s protocol (Stratagene, La Jolla, CA) for digestion (EcoRI/NotI), and ligated into pGENE-V5-C (Invitrogen, Carlsbad, CA) to construct pGENE-KC-V5-C. Intron II of the rabbit β-globin gene was PCR amplified from pSG5-KHA2M1 (Stratagene), gel-purified, shuttled through pPCRScript for digestion (BstX1), ligated into pGENE-KC-V5-C to construct pGENE-intron-KC-V5-C, and verified by sequencing with the pGENE forward primer (Stratagene). .. The intron-KC-V5 fragment was PCR amplified, with flanking SpeI sites, gel-purified, shuttled through pPCRScript for digestion (SpeI), ligated into pBSIISK-ARR 2 PB to construct pBSIISK-ARR 2 PB-intron-KC-V5, and verified by sequencing using the T7 primer (Promega, Madison, WI).

    Article Title: Influence of parasite encoded inhibitors of serine peptidases in early infection of macrophages with Leishmania major
    Article Snippet: .. The PCR product was subcloned into pPCRscript (Stratagene). ..

    Article Title: Dual Targeting of Arabidopsis HOLOCARBOXYLASE SYNTHETASE1: A Small Upstream Open Reading Frame Regulates Translation Initiation and Protein Targeting
    Article Snippet: .. The resulting PCR products were cloned into pPCRscript (Stratagene) and sequenced. .. Cloning of HCS1 cDNA Splicing Variants Poly(A) mRNAs from Arabidopsis rosette leaves were prepared using the Straight A's mRNA isolation system according to the manufacturer's instructions (Novagen), followed by a treatment with RNase-free DNase I. First-strand cDNA was synthesized from 250 ng of DNA-free mRNA in a final volume of 20 μ L using oligo(dT) 20 primers (Thermoscript RT-PCR system; Life Technologies).

    Article Title: Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.
    Article Snippet: .. Furthermore, a variant adapted to the codon usage of highly expressed mammalian genes (mEPOopt) was designed using the proprietary GeneOptimizer software (Geneart AG, Regensburg, Germany).57 All mEPO sequences were generated by a PCR-based method and were cloned into pPCRScript (Stratagene, La Jolla, CA, USA) via KpnI and SacI restriction sites. .. The eukaryotic expression vector pRS was generated by deleting the FRT/hygromycin cassette from the commercial expression vector pcDNA5 (Invitrogen, Karlsruhe, Germany) via PCR using oligonucleotides 5¢-T CAGATGCATCCGTACGTTAACATGTGAGCAAAAGGCCAGCA-3¢ and 5¢-AG TCATGCATCCATAGAGCCCACCGCATCCCCA-3¢.

    Article Title: Isolation of Paenibacillus glucanolyticus from pulp mill sources with potential to deconstruct pulping waste.
    Article Snippet: Black liquor is a pulping waste generated by the kraft process that has potential for downstream bioconversion.. A microorganism was isolated from a black liquor sample collected from the Department of Forest Biomaterials at North Carolina State University.. The organism was identified as Paenibacillus glucanolyticus using 16S rRNA sequence analysis and was shown to be capable of growth on black liquor as the sole carbon source based on minimal media growth studies.

    Amplification:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: The enhanced probasin promoter, ARR 2 PB (courtesy of Dr. Robert Matusik) [ 34 , 35 ], was cloned into the MCS of the pBSIISK subcloning vector (Stratagene, La Jolla, CA) to construct pBSIISK-ARR 2 PB. .. Mouse KC, from the Kozak secretion signal through the penultimate amino acid, was PCR amplified from the IMAGE consortium clone {"type":"entrez-nucleotide","attrs":{"text":"BC037997","term_id":"23958859","term_text":"BC037997"}} BC037997 (Open BioSystems, Huntsville, AL), gel-purified (GeneClean kit, Qbiogene, Irvine, CA), shuttled through pPCRScript according to the manufacturer’s protocol (Stratagene, La Jolla, CA) for digestion (EcoRI/NotI), and ligated into pGENE-V5-C (Invitrogen, Carlsbad, CA) to construct pGENE-KC-V5-C. Intron II of the rabbit β-globin gene was PCR amplified from pSG5-KHA2M1 (Stratagene), gel-purified, shuttled through pPCRScript for digestion (BstX1), ligated into pGENE-KC-V5-C to construct pGENE-intron-KC-V5-C, and verified by sequencing with the pGENE forward primer (Stratagene). .. The intron-KC-V5 fragment was PCR amplified, with flanking SpeI sites, gel-purified, shuttled through pPCRScript for digestion (SpeI), ligated into pBSIISK-ARR 2 PB to construct pBSIISK-ARR 2 PB-intron-KC-V5, and verified by sequencing using the T7 primer (Promega, Madison, WI).

    Article Title: Synapsins are late activity-induced genes regulated by birdsong
    Article Snippet: .. The amplified product was excised from an agarose gel, eluted using Qiagen Gel Extraction Kit® (Qiagen Inc, Valencia, CA, USA), inserted into pPCRScript (Stratagene Inc., La Jolla, CA, USA) and used to transform bacterial cells following standard procedures. .. Insert identity was confirmed by sequencing and analysis using DNAStar software (DNAStar Inc., Madison, WI, USA).

    Construct:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: The enhanced probasin promoter, ARR 2 PB (courtesy of Dr. Robert Matusik) [ 34 , 35 ], was cloned into the MCS of the pBSIISK subcloning vector (Stratagene, La Jolla, CA) to construct pBSIISK-ARR 2 PB. .. Mouse KC, from the Kozak secretion signal through the penultimate amino acid, was PCR amplified from the IMAGE consortium clone {"type":"entrez-nucleotide","attrs":{"text":"BC037997","term_id":"23958859","term_text":"BC037997"}} BC037997 (Open BioSystems, Huntsville, AL), gel-purified (GeneClean kit, Qbiogene, Irvine, CA), shuttled through pPCRScript according to the manufacturer’s protocol (Stratagene, La Jolla, CA) for digestion (EcoRI/NotI), and ligated into pGENE-V5-C (Invitrogen, Carlsbad, CA) to construct pGENE-KC-V5-C. Intron II of the rabbit β-globin gene was PCR amplified from pSG5-KHA2M1 (Stratagene), gel-purified, shuttled through pPCRScript for digestion (BstX1), ligated into pGENE-KC-V5-C to construct pGENE-intron-KC-V5-C, and verified by sequencing with the pGENE forward primer (Stratagene). .. The intron-KC-V5 fragment was PCR amplified, with flanking SpeI sites, gel-purified, shuttled through pPCRScript for digestion (SpeI), ligated into pBSIISK-ARR 2 PB to construct pBSIISK-ARR 2 PB-intron-KC-V5, and verified by sequencing using the T7 primer (Promega, Madison, WI).

    Sequencing:

    Article Title: Keratinocyte-Derived Chemokine Induces Prostate Epithelial Hyperplasia and Reactive Stroma in a Novel Transgenic Mouse Model
    Article Snippet: The enhanced probasin promoter, ARR 2 PB (courtesy of Dr. Robert Matusik) [ 34 , 35 ], was cloned into the MCS of the pBSIISK subcloning vector (Stratagene, La Jolla, CA) to construct pBSIISK-ARR 2 PB. .. Mouse KC, from the Kozak secretion signal through the penultimate amino acid, was PCR amplified from the IMAGE consortium clone {"type":"entrez-nucleotide","attrs":{"text":"BC037997","term_id":"23958859","term_text":"BC037997"}} BC037997 (Open BioSystems, Huntsville, AL), gel-purified (GeneClean kit, Qbiogene, Irvine, CA), shuttled through pPCRScript according to the manufacturer’s protocol (Stratagene, La Jolla, CA) for digestion (EcoRI/NotI), and ligated into pGENE-V5-C (Invitrogen, Carlsbad, CA) to construct pGENE-KC-V5-C. Intron II of the rabbit β-globin gene was PCR amplified from pSG5-KHA2M1 (Stratagene), gel-purified, shuttled through pPCRScript for digestion (BstX1), ligated into pGENE-KC-V5-C to construct pGENE-intron-KC-V5-C, and verified by sequencing with the pGENE forward primer (Stratagene). .. The intron-KC-V5 fragment was PCR amplified, with flanking SpeI sites, gel-purified, shuttled through pPCRScript for digestion (SpeI), ligated into pBSIISK-ARR 2 PB to construct pBSIISK-ARR 2 PB-intron-KC-V5, and verified by sequencing using the T7 primer (Promega, Madison, WI).

    Agarose Gel Electrophoresis:

    Article Title: Synapsins are late activity-induced genes regulated by birdsong
    Article Snippet: .. The amplified product was excised from an agarose gel, eluted using Qiagen Gel Extraction Kit® (Qiagen Inc, Valencia, CA, USA), inserted into pPCRScript (Stratagene Inc., La Jolla, CA, USA) and used to transform bacterial cells following standard procedures. .. Insert identity was confirmed by sequencing and analysis using DNAStar software (DNAStar Inc., Madison, WI, USA).

    Article Title: Isolation of Paenibacillus glucanolyticus from pulp mill sources with potential to deconstruct pulping waste.
    Article Snippet: Black liquor is a pulping waste generated by the kraft process that has potential for downstream bioconversion.. A microorganism was isolated from a black liquor sample collected from the Department of Forest Biomaterials at North Carolina State University.. The organism was identified as Paenibacillus glucanolyticus using 16S rRNA sequence analysis and was shown to be capable of growth on black liquor as the sole carbon source based on minimal media growth studies.

    Gel Extraction:

    Article Title: Synapsins are late activity-induced genes regulated by birdsong
    Article Snippet: .. The amplified product was excised from an agarose gel, eluted using Qiagen Gel Extraction Kit® (Qiagen Inc, Valencia, CA, USA), inserted into pPCRScript (Stratagene Inc., La Jolla, CA, USA) and used to transform bacterial cells following standard procedures. .. Insert identity was confirmed by sequencing and analysis using DNAStar software (DNAStar Inc., Madison, WI, USA).

    Clone Assay:

    Article Title: Dual Targeting of Arabidopsis HOLOCARBOXYLASE SYNTHETASE1: A Small Upstream Open Reading Frame Regulates Translation Initiation and Protein Targeting
    Article Snippet: .. The resulting PCR products were cloned into pPCRscript (Stratagene) and sequenced. .. Cloning of HCS1 cDNA Splicing Variants Poly(A) mRNAs from Arabidopsis rosette leaves were prepared using the Straight A's mRNA isolation system according to the manufacturer's instructions (Novagen), followed by a treatment with RNase-free DNase I. First-strand cDNA was synthesized from 250 ng of DNA-free mRNA in a final volume of 20 μ L using oligo(dT) 20 primers (Thermoscript RT-PCR system; Life Technologies).

    Article Title: Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.
    Article Snippet: .. Furthermore, a variant adapted to the codon usage of highly expressed mammalian genes (mEPOopt) was designed using the proprietary GeneOptimizer software (Geneart AG, Regensburg, Germany).57 All mEPO sequences were generated by a PCR-based method and were cloned into pPCRScript (Stratagene, La Jolla, CA, USA) via KpnI and SacI restriction sites. .. The eukaryotic expression vector pRS was generated by deleting the FRT/hygromycin cassette from the commercial expression vector pcDNA5 (Invitrogen, Karlsruhe, Germany) via PCR using oligonucleotides 5¢-T CAGATGCATCCGTACGTTAACATGTGAGCAAAAGGCCAGCA-3¢ and 5¢-AG TCATGCATCCATAGAGCCCACCGCATCCCCA-3¢.

    Article Title: Isolation of Paenibacillus glucanolyticus from pulp mill sources with potential to deconstruct pulping waste.
    Article Snippet: Black liquor is a pulping waste generated by the kraft process that has potential for downstream bioconversion.. A microorganism was isolated from a black liquor sample collected from the Department of Forest Biomaterials at North Carolina State University.. The organism was identified as Paenibacillus glucanolyticus using 16S rRNA sequence analysis and was shown to be capable of growth on black liquor as the sole carbon source based on minimal media growth studies.

    Variant Assay:

    Article Title: Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.
    Article Snippet: .. Furthermore, a variant adapted to the codon usage of highly expressed mammalian genes (mEPOopt) was designed using the proprietary GeneOptimizer software (Geneart AG, Regensburg, Germany).57 All mEPO sequences were generated by a PCR-based method and were cloned into pPCRScript (Stratagene, La Jolla, CA, USA) via KpnI and SacI restriction sites. .. The eukaryotic expression vector pRS was generated by deleting the FRT/hygromycin cassette from the commercial expression vector pcDNA5 (Invitrogen, Karlsruhe, Germany) via PCR using oligonucleotides 5¢-T CAGATGCATCCGTACGTTAACATGTGAGCAAAAGGCCAGCA-3¢ and 5¢-AG TCATGCATCCATAGAGCCCACCGCATCCCCA-3¢.

    Software:

    Article Title: Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.
    Article Snippet: .. Furthermore, a variant adapted to the codon usage of highly expressed mammalian genes (mEPOopt) was designed using the proprietary GeneOptimizer software (Geneart AG, Regensburg, Germany).57 All mEPO sequences were generated by a PCR-based method and were cloned into pPCRScript (Stratagene, La Jolla, CA, USA) via KpnI and SacI restriction sites. .. The eukaryotic expression vector pRS was generated by deleting the FRT/hygromycin cassette from the commercial expression vector pcDNA5 (Invitrogen, Karlsruhe, Germany) via PCR using oligonucleotides 5¢-T CAGATGCATCCGTACGTTAACATGTGAGCAAAAGGCCAGCA-3¢ and 5¢-AG TCATGCATCCATAGAGCCCACCGCATCCCCA-3¢.

    Generated:

    Article Title: Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.
    Article Snippet: .. Furthermore, a variant adapted to the codon usage of highly expressed mammalian genes (mEPOopt) was designed using the proprietary GeneOptimizer software (Geneart AG, Regensburg, Germany).57 All mEPO sequences were generated by a PCR-based method and were cloned into pPCRScript (Stratagene, La Jolla, CA, USA) via KpnI and SacI restriction sites. .. The eukaryotic expression vector pRS was generated by deleting the FRT/hygromycin cassette from the commercial expression vector pcDNA5 (Invitrogen, Karlsruhe, Germany) via PCR using oligonucleotides 5¢-T CAGATGCATCCGTACGTTAACATGTGAGCAAAAGGCCAGCA-3¢ and 5¢-AG TCATGCATCCATAGAGCCCACCGCATCCCCA-3¢.

    other:

    Article Title: Influence of parasite encoded inhibitors of serine peptidases in early infection of macrophages with Leishmania major
    Article Snippet: Phosphostorage screens were exposed overnight and the membranes visualized using a Typhoon 8610 variable mode imager (Molecular Dynamics).

    Process/Product Development:

    Article Title: Isolation of Paenibacillus glucanolyticus from pulp mill sources with potential to deconstruct pulping waste.
    Article Snippet: Black liquor is a pulping waste generated by the kraft process that has potential for downstream bioconversion.. A microorganism was isolated from a black liquor sample collected from the Department of Forest Biomaterials at North Carolina State University.. The organism was identified as Paenibacillus glucanolyticus using 16S rRNA sequence analysis and was shown to be capable of growth on black liquor as the sole carbon source based on minimal media growth studies.



    Similar Products

    90
    Agilent technologies ppcrscript
    Ppcrscript, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/pm24841577-73-18-19
    Average 90 stars, based on 1 article reviews
    ppcrscript - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies ppcrscript amp sk(+) vector
    Ppcrscript Amp Sk(+) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/pm25417903-144-37-49
    Average 90 stars, based on 1 article reviews
    ppcrscript amp sk(+) vector - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies ppcrscript cam sk
    Ppcrscript Cam Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/us08889709-834-6-11
    Average 90 stars, based on 1 article reviews
    ppcrscript cam sk - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies ppcrscript amp sk
    Ppcrscript Amp Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/pmc03174548-92-12-17
    Average 90 stars, based on 1 article reviews
    ppcrscript amp sk - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Thermo Fisher ppcrscript-e (codon optimized) plasmid
    Ppcrscript E (Codon Optimized) Plasmid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/DreamTaq+Hot+Start+Green+PCR+Master+Mix/10__1091_slash_mbc__e10___04___0338-68-1-5
    Average 90 stars, based on 1 article reviews
    ppcrscript-e (codon optimized) plasmid - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    90
    Agilent technologies ppcrscript vector
    Ppcrscript Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/us07769575-134-5-7
    Average 90 stars, based on 1 article reviews
    ppcrscript vector - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    96
    TaKaRa ppcrscript vector
    Ppcrscript Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppcrscript/pmCherry+Vector/pmc07115638-66-14-16
    Average 96 stars, based on 1 article reviews
    ppcrscript vector - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    Image Search Results